Gene expression control in germ cells
The germline is an immortal cell lineage tasked with propagating the highest quality DNA. Germ cells regulate mutation and recombination rates, suppress selfish genetic elements like transposons, and produce gametes. The germline also gives rise to multiple somatic cell lineages to help it acquire nutrients and reproduce within a highly competitive and changing environment (the world). Perhaps to juggle its myriad functions, germ cells regulate genes differently than highly specialized somatic cells. Compared to most somatic cells, germ cell gene expression is more permissive but less robust. Most germ cells lack strong transcriptional activators and repressors and germ cell chromatin is more dispersed and accessible. We study how and why germ cells adopt this unique transcriptional state using a variety of tools we developed to purify and genetically manipulate germ cells in the experimentally tractable Drosophila ovary.
Developmental regulation of gene silencing
As somatic cells differentiate, they inhibit the transcription of many genes they will no longer need for their specialized purposes. This developmentally-regulated gene silencing is triggered and maintained by Polycomb-Group proteins. Beginning with genetic screens in flies, many Polycomb-Group proteins have been identified, but how they work together to silence the correct genes at the right time and place in development is poorly understood.
We developed a new model system, Drosophila nurse cell differentiation, to study these questions. Our system allows us to precisely manipulate Polycomb Group proteins and study their function during silencing initiation and maintenance using microscopy, molecular biology, and genomics. Using our nurse cell differentiation system, we discovered how two Polycomb proteins, Scm and Pcl, regulate the initiation of two types of gene silencing. Read more about it here
We are located in the Cooley Lab building on MSU-Bozeman’s main campus.
225 Cooley Lab Building
Just off of S. 11th Ave, north of the MSU Duck Pond
- DeLuca et al. (2020)Differentiating Drosophila female germ cells initiate Polycomb silencing by regulating PRC2-interacting proteins
- DeLuca et al. (2018)Efficient Expression of Genes in the Drosophila Germline Using a UAS-Promoter Free of Interference by Hsp70 piRNAs
- Yu et al. (2017)The Mitochondrial DNA Polymerase Promotes Elimination of Paternal Mitochondrial Genomes
- Nagarkar-Jaiswal et al. (2015)A genetic toolkit for tagging intronic MiMIC containing genes
- DeLuca et al. (2012)Barriers to Male Transmission of Mitochondrial DNA in Sperm Development
- Foe et al. (2011)Ubiquitination of Cdc20 by the APC Occurs through an Intramolecular Mechanism
- Xu et al. (2008)Manipulating the metazoan mitochondrial genome with targeted restriction enzymes
- Au et al. (2008)Altered dosage and mislocalization of histone H3 and Cse4p lead to chromosome loss in Saccharomyces cerevisiae
January 2022: The DeLuca Lab opens at Brandeis University!
May 2023: Congratulations to Tian Tan for brilliantly defending her undergraduate thesis! Best of luck pursuing your doctorate at Johns Hopkins!
May 2023: Lab moves to MSU-Bozeman! Go cats!